Vasodilator-Stimulated Phosphoprotein Activity Is Required for Coxiella burnetii Growth in Human Macrophages
Fig 5
VASP activity is required for optimal PV formation.
THP-1 cells were transfected with non-targeting (NT) or VASP-specific siRNA and knockdown confirmed by immunoblot. Cells were processed for confocal microscopy at 72 hpi. DNA was labeled with DAPI (blue), and CD63 (green) and C. burnetii (red) were detected with antibodies. Bar, 10 μm. The scatter plot displays PV diameter in NT or VASP siRNA-transfected cells. PV measurements were taken from at least 15 randomly selected fields. When multiple vacuoles were visible, the two largest vacuoles were measured in a single cell. The horizontal bar indicates average vacuole size. * indicates p < 0.0001 according to a Student’s t test comparing vacuole size in NT and VASP siRNA-transfected cells. VASP knockdown results in formation of smaller PV.