Identification of Novel Rosavirus Species That Infects Diverse Rodent Species and Causes Multisystemic Dissemination in Mouse Model
Fig 5
Western blot analysis for detection of antibodies against purified His6-tagged recombinant VP1 proteins of rosavirus B strain RNCW1002091R from a Norway rat and rosavirus C strains, RASK8F from an Indochinese forest rat and NCGX12IN from a Coxing's white-bellied rat (~40-kDa) in rodent serum samples.
Representatives of results are shown. Lanes: 1, Norway rat serum sample positive for antibody against rosavirus B strain RNCW1002091R VP1 protein; 2, Indochinese forest rat serum sample positive for antibody against rosavirus C strain RASK8F VP1 protein; 3, Coxing's white-bellied rat serum sample positive for antibody against rosavirus C strain NCGX12IN VP1 protein; 4, rosavirus B-VP1 antibody-positive Norway rat serum sample against rosavirus C strain NCGX12IN VP1 protein; 5, rosavirus C-VP1 antibody-positive Coxing's white-bellied rat serum sample against rosavirus B strain RNCW1002091R VP1 protein; 6, positive control (anti-His antibody); 7, negative control.