Fis Is Essential for Yersinia pseudotuberculosis Virulence and Protects against Reactive Oxygen Species Produced by Phagocytic Cells during Infection
Fig 5
ΔdusB-fis can colonize, but is unable to sustain growth in systemic tissue sites.
C57BL6/J mice were inoculated intravenously with a pool of 103 bacteria, containing either an equal mixture of WT yopE::mcherry and ΔdusB-fis-KanR (A-B), or with WT yopE::mcherry only or ΔdusB-fis-KanR only (C-D). At 4-hour, 24-hour, 48-hour, and 72-hour time-points post-infection, mice were euthanized and spleens and livers were collected. Dilutions of liver (A, C) and spleen (B, D) homogenates were plated and CFU calculated. In co-infection experiments (A-B), the number of bacteria recovered from selective and non-selective plates was used to determine quantity of WT yopE::mcherry and ΔdusB-fis-KanR bacteria in each organ sample. CFU/g data was log10 transformed and the values of ΔdusB-fis-KanR to WT yopE::mcherry bacteria in each tissue at each time point was compared using an unpaired t-test with the Holm-Sidak correction for multiple comparisons. * indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001, and **** indicates p≤0.0001.