Latency Entry of Herpes Simplex Virus 1 Is Determined by the Interaction of Its Genome with the Nuclear Environment
Fig 5
Type 1 IFNα induces an ML-like pattern in infected cultured primary mouse TG neurons.
(A) Immuno-FISH for the detection of PML and HSV-1 genomes in cells not treated (i) or treated (ii) with IFNα (1000 IU/mL). (B) Double DNA FISH for the detection of centromeric minor satellite sequences and HSV-1 genomes in cells treated with IFNα (1000 IU/mL). (C) Quantification of ML-like and RC patterns in neurons (three independent experiments) infected with in1374, in1330, or tsK for 24 h in the absence or presence of IFNα (1000 IU/mL). Results show means (± SD). (D) Quantification of ML-like and RC patterns in neurons from C57BL/6 wt or IFNAR KO mice, and infected by in1374 for 24 h in the absence or presence of IFNα (1000 IU/mL). Means of two independent experiments are shown.