Polarized Cell Division of Chlamydia trachomatis
Fig 5
Characterization of the polarized division process of Chlamydia in more mature inclusions.
HeLa cells were infected with C. trachomatis and fixed at 14 hours post-infection. The cells were then permeabilized and incubated with anti-MOMP goat polyclonal antibodies (green) and anti-IncG rabbit polyclonal antibodies (red) followed by donkey anti-goat IgG conjugated to Alexa Fluor 488 and donkey anti-rabbit IgG conjugated to Alexa Fluor 568 secondary antibodies (A and B). The cells were then washed and stained with Hoechst 33342 (blue) prior to confocal analysis. The images in B are consecutive confocal slices from a z-stack. Arrowheads in A and B point to sites of asymmetric membrane expansion. Asterisk in B indicates a cell simultaneously undergoing polar growth from two sites. The white bars are 0.5μ. The numbers in the merged images in B correspond to the position in the Z-stack in microns.