Two Different Virulence-Related Regulatory Pathways in Borrelia burgdorferi Are Directly Affected by Osmotic Fluxes in the Blood Meal of Feeding Ixodes Ticks
Fig 5
Rrp1 is required for the survival of B. burgdorferi at higher osmolarity.
(A) Rate of growth of B31-5A4, B31-5A4Δhk1 and B31-5A4Δrrp1 mutants at osmolarities ranging from 150 to 1050 mOsm under microaerobic growth conditions. (B) Growth of strain B31-5A4 and 5A4Δrrp1 mutant in BSK-II at various osmolarities. Cells were quantified by colonies on BSK-II plating media. (C) hk1 expression was analyzed by relative qRT-PCR in B. burgdorferi B31-A3 normalized to enoS. (D) Quantitation of c-di-GMP in B31-5A4, B31-5A4Δhk1 and B31-5A4Δrrp1 mutants.