Trypanosoma brucei Co-opts NK Cells to Kill Splenic B2 B Cells
Fig 5
Splenic B cell populations in uninfected and T. brucei AnTat 1.1-infected TCR-/- C57BL/6 mice.
Splenic B cells in uninfected B6-TCR-/- mice (n = 3) and B6-TCR-/- (n = 3) mice that had been infected with T. brucei AnTat 1.1 for 10 days were stained for surface markers used to define transitional and mature B2 B cells as described in Table 1 and analyzed using flow cytometry. Results are presented as individual and mean +/- 1SD numbers of: (A) Transitional 2+3 B cells/spleen, (B) MZB cells/spleen and (C) FoB cells/spleen. Significance (*p<0.05; **p<0.01) is determined using one-way ANOVA and Tukey’s HSD test comparing uninfected controls to infected individuals. Results are representative of 3 identical experiments. D–G Representative FACS plots of: (D) FoB and MZB spleen cells from an uninfected TCR-/- C57BL/6 mouse, (E) FoB and MZB spleen cells from a day 10 infected TCR-/- C57BL/6 mouse, (F) Transitional (AA4.1+, B220+) spleen cells from an uninfected TCR-/- C57BL/6, (G) Transitional (AA4.1+, B220+) spleen cells from an a day 10 infected TCR-/- C57BL/6 mouse.