The WOPR Protein Ros1 Is a Master Regulator of Sporogenesis and Late Effector Gene Expression in the Maize Pathogen Ustilago maydis
Fig 2
ros1 deletion strains fail to aggregate and form spores.
Tumor samples from maize seedlings infected with the wild type strains FB1 x FB2 and the corresponding ros1 deletion strains were collected at 4, 6, 8, 10, 12 dpi, stained with wheat germ agglutinin-Alexa Fluor 488 and analyzed by laser scanning confocal microscopy (bar = 50 μm). In the FB1 x FB2 infection (left column), spore formation starts at 6 dpi with the formation of hyphal aggregates which expand (8 dpi), begin to fragment (10 dpi) and develop into teliospores (12 dpi). In comparison, the ros1 deletion strains are locked in the filamentous state, do not aggregate and do not develop teliospores (right column). The size bar corresponds to 50 μM.