An Oncogenic Virus Promotes Cell Survival and Cellular Transformation by Suppressing Glycolysis
Fig 5
Knock down of RelA increases the expression of GLUT1 and GLUT3, and aerobic glycolysis.
(A). Analysis of RelA, GLUT1 and GLUT3 proteins in MM and KMM cells by Western-blot following knock down of RelA. Detection of RelA, GLUT1 and GLUT3 proteins in MM and KMM cells following transfection with a siRNA to RelA (siRelA) or a scrambled control (Scr) for 3 days. β-tubulin was used as an internal control for loading. (B-C). Analysis of RelA (B), GLUT1 (C) and GLUT3 (D) mRNAs in MM and KMM cells by RT-qPCR following knock down of RelA. Cells were treated as described in (A). β-actin was used as an internal control for qPCR. (E). Knock down of RelA decrease cell proliferation of KMM but not MM cells. Cell proliferation were examined following knock down of RelA. (F-G) Knock down of RelA increases glucose consumption (F) and lactate production (G) in KMM cells. Glucose consumption and lactate production were determined as described in Fig 1C and 1E following knock down of RelA. Experiments were repeated three times, each with three repeats and representative results were presented. * P < 0.05; ** P < 0.01; *** P < 0.001.