Pathogenic Chlamydia Lack a Classical Sacculus but Synthesize a Narrow, Mid-cell Peptidoglycan Ring, Regulated by MreB, for Cell Division
Fig 5
C. trachomatis incorporates DAADs rapidly during exponential growth.
(a) SIM of chlamydial inclusions (18 hpi) incubated for 10 min with 4 mM EDA—DA. Z-stacks from chlamydial inclusions (18 hpi) incubated with EDA—DA were collected, maximum intensity projections constructed, and average pixel intensities calculated for entire inclusions for both MOMP (b) and EDA—DA (c) channels. ~200 inclusions were assigned average pixel intensities per time point measured and the entire experiment was carried out in biological duplicates. MOMP and PG labeling is as presented in Fig 2. Scale bar = 1 μm.