Entamoeba histolytica Cysteine Proteinase 5 Evokes Mucin Exocytosis from Colonic Goblet Cells via αvβ3 Integrin
Fig 2
αvβ3 is the critical CP5 receptor on goblet cells responsible for mucin secretion.
Mucins from LS174T goblet cells were metabolically labeled with 3H-glucosamine and assayed for mucins secretion in the presence of A, GRGDSP (50μM) and B, αvβ3 function-blocking antibody (LM609 5μg/mL) for 2 h with live Eh. These were compared to the appropriate controls GRADSP and mouse IgG respectively. C. Eh induced mucin secretion in the presence of a specific αvβ3 inhibitor P11 (10 μM) with either GRADSP (50μM) or GRGDSP (50μM). D. αvβ3 binding to various adhesive proteins as assessed by ELISA. Vitronectin and laminin are the positive and negative controls respectively. For P11 inhibition, the inhibitor was included when αvβ3 was added and used at a concentration of 1μM. ***p <0.001, **p <0.01, *p <0.05.