The Ancient Evolutionary History of Polyomaviruses
Fig 2
Structural modeling of LT proteins.
The solved OBD-Zn-ATPase SV40 LT structure (PDB identifier 4GDF) was used as template for all OBD and Zn-ATPase domain models. The model of the guitarfish polyomavirus J domain was generated using the solved structure of the SV40 LT DNAJ domain (PDB identifier 1GH6) as template. For the DNAJ domain of scorpion polyomavirus LT, the best modeling template match is a Thermus thermophilus DNAJ protein (PDB identifier 4J7Z). The solved structure of the bacterial DNAJ is highlighted in magenta in the pairwise superimposition (top left). The LT proteins of the indicated polyomavirus species are shown in black. The known structures of SV40 LT domains are superimposed in gold. The conserved HPD motif of the DNAJ domain is positioned on the top and highlighted in cyan. The N-terminal domain of the notothen polyomavirus has no discernible structural similarity to known DNAJ structures.