PPARγ Is Activated during Congenital Cytomegalovirus Infection and Inhibits Neuronogenesis from Human Neural Stem Cells
Fig 5
Soluble mediators from infected NSCs trigger the expression of PPARγ in uninfected NSCs.
(A) Immunofluorescence analysis using antibodies specific to IE or PPARγ showing strong increase in PPARγ levels in NSCs infected by HCMV (HCMV), or in uninfected NSCs treated with supernatants prepared from HCMV-infected NSC cultures (SN-HCMV), as compared to uninfected NSCs treated with standard growth medium (mock), or NSCs treated with supernatants prepared from uninfected NSC cultures (SN-NI). The asterisk points to a representative cell with PPARγ nuclear staining. (B) Immunofluorescence analysis using antibodies specific to nestin or PPARγ showing strong increase in PPARγ levels in uninfected NSCs exposed to lipid extracts purified from the supernatants of HCMV-infected NSCs cultures (L-HCMV), as compared to NSCs exposed to lipid extracts purified from the supernatants of uninfected NSCs cultures (L-NI). Virus strain was AD169, and the MOI was 10 in all cases. Scale bar: 50 μm.