Candida glabrata Binding to Candida albicans Hyphae Enables Its Development in Oropharyngeal Candidiasis
Fig 5
C. albicans and C. glabrata are co-localized and both invade murine tongue tissues in a mixed infection.
(A, B and C) PAS stained formalin-fixed and paraffin embedded sections (5 μm) from tongues of mixed C. albicans and C. glabrata infection at day 5 showed widespread fungal plaques and hyphal invasion (yeast are stained magenta) of superficial epithelium (box) and underlying epithelium (arrows). Dark blue cells are neutrophils, lighter blue cells are tongue epithelia. Magnification is 10x. (B) 40x and (C) 100x magnification show C. albicans hyphae with associated yeast cells within the mucosa. (D, E and F) Immunofluorescent and DIC merged images of C. albicans (red) and C. glabrata (green) from tongues at day 5 post-infection. (D) Arrowheads show hyphae penetration into the epithelium. (E and F) Arrowheads at left indicate C. glabrata in contact with hyphae, while other C. glabrata are within epithelium unassociated with C. albicans hyphae (E, lower arrow). Scale bars represent, in order (A-F), 50, 10, 5, 50, 10, and 10 μm.