Influenza Virus Targets Class I MHC-Educated NK Cells for Immunoevasion
Fig 6
Involvement of Ly49 inhibitory receptors in the pathogenesis of influenza virus.
(A) Groups of influenza-infected age and sex-matched WT, NKCKD, and littermate NKCKD-Ly49Itg mice were infected with 600 PFU of FM-MA virus, then monitored as before. Data are pooled from two independent experiments (n = 16 in each group). (B) Groups of influenza-infected age and sex-matched WT (B6) and B2m-/- mice were monitored as in Fig 1. One group each of WT or B2m-/- mice was treated with 200 μg anti-NK1.1 mAb per mouse i.p. two days prior to influenza virus infection, on the day of infection (600 PFU), and every two days after until day 10 p.i. Data are pooled from two independent experiments (n = 10 in each group). (C) Groups of age and sex-matched WT (B6) mice were treated or not with 200 μg of 5E6 F(ab')2 mAbs per mouse i.p. two days prior to influenza virus infection (600 PFU), on the day of infection, and every two days post-influenza virus infection until day 10 p.i. Data are pooled from two independent experiments (n = 16 in each group). *p < 0.05, **p < 0.01 and ***p < 0.001. Statistical analysis was performed with the log rank test.