Influenza Virus Targets Class I MHC-Educated NK Cells for Immunoevasion
Fig 5
Lymphocyte recruitment to the lungs following influenza virus infection.
WT and NKCKD mice were infected intranasally with 600 PFU of FM-MA virus. Single-cell suspensions were prepared from uninfected lungs and those infected with the virus for 5 or 7 days. The following mAb were used in this experiment: anti-CD8, anti-CD3, anti-CD4, and anti-NKp46 mAb; as well, Kb-NS2114−121 tetramers and I-Ab–NP311-325 tetramers were used. Both the frequency (%) and absolute number (#) of cell populations is reported. (A, B) NS2114-121 CD8+ specific T cells were gated out of CD3+ CD8+ T cells. (C, D) NP311-325 CD4+specific T cells were gated out of CD3+ CD4+ T cells. (E, F) NK cells were detected as CD3- NKp46+ cells. Data are pooled from two independent experiments (n = 6 in each group). Horizontal bars represent mean values. Each symbol represents data from a single mouse. *p < 0.05 and **p < 0.01. Statistical analysis was performed using Student’s t-test.