Influenza Virus Targets Class I MHC-Educated NK Cells for Immunoevasion
Fig 2
Increased numbers of activated, uneducated NK cells correlates with protection from influenza.
Groups of age and sex-matched WT mice were infected as in Fig 1. On day 5 p.i., mice were sacrificed, and single cell suspensions prepared from the lungs. Flow cytometry was performed as above, using cells stained with a fixable viability dye, anti-NKp46, anti-TCRβ, anti-Ly49C/I (5E6), anti-Ly49G (4D11), anti-Ki-67, anti-IFN-γ, and anti-CD107a mAbs. Each symbol represents one mouse. Cell counts are given in absolute number calculated per lung. Horizontal bars represent mean values, *p < 0.05, **p < 0.01, and ***p < 0.001. Statistical analysis was performed using a one-way ANOVA followed by a Bonferroni post-hoc test.