The ER-Membrane Transport System Is Critical for Intercellular Trafficking of the NSm Movement Protein and Tomato Spotted Wilt Tospovirus
Fig 5
Mutations in NSm that blocked correct ER sorting or altered sorting to other subcellular localization inhibits NSm cell-to-cell movement in leaf epidermis of N. benthamiana.
(A-C) Subcellular localization of NSm4A/5A mutant in epidermal cell. ER marker mCherry-HDEL was used for colocalization analysis. Bar, 10 μm. (D-F) Colocalization analysis of NSm230A/232A mutant with ER labeled by mCherry-HDEL. Bar, 10 μm. (G-I) Cell-to-cell movement analysis of NSm4A/5A (H) and NSm230A/232A (I) mutants in N. benthamiana after bombardment. NSmWT (G) was used as a positive control. Bar, 50 μm.