HTLV-1 bZIP Factor Impairs Anti-viral Immunity by Inducing Co-inhibitory Molecule, T Cell Immunoglobulin and ITIM Domain (TIGIT)
Fig 5
HBZ suppresses Fgl2 and CD226 expression.
(A) Fgl2 mRNA was quantified in HBZ-expressing murine primary CD4+ T cells and control cells by realtime PCR. This experiment was carried out in triplicate, and the mean values ± SD are shown. Three independent experiments were performed and the representative results are shown. (B) The levels of Fgl2 mRNA were measured in CD4+ T cells from non-Tg (n = 4) and TIGIT+CD4+ and TIGIT-CD4+ T cells from HBZ-Tg (n = 3) mice. (C) Jurkat cells were cotransfected with 500 ng of FGL2-promoter-Luc, pME18Sneo-sHBZ, 500 ng of pcDNA-C/EBPα and pGL4-TK. Luciferase activity was measured after 24 hours. Luciferase assay was carried out in triplicate, and the mean values ± SD are shown. Three independent experiments were performed and the representative results were shown. (D) The CD226 gene transcript was quantified in HBZ-expressing murine primary CD4+ T cells and control cells. This assay was carried out in triplicate and the mean values ± SD are shown. Three independent experiments were performed and the representative results were shown. (E) The CD226 gene expression was measured in CD4+ T cells from HBZ-Tg (n = 7) and non-Tg (n = 7) mice by realtime PCR. *P < 0.05, **P < 0.01.