An Oomycete CRN Effector Reprograms Expression of Plant HSP Genes by Targeting their Promoters
Fig 2
PsCRN108 contributes to the virulence of P. sojae.
In all panels, WT = wild type P6497; T17 is a non-silenced transformant; ST18 and ST19 are PsCRN108-silenced transformants. (A) Relative transcript levels of PsCRN108 in different transgenic P. sojae lines. The transcript levels of the PsCRN108 and PsCRN112a/b/c genes were measured by qRT-PCR and normalized to those in the WT using the actin gene as an internal reference. Bars represent standard errors from three independent biological replicates (**, P<0.01 compared with the WT; Dunnett's test). (B) Lengths of lesions on etiolated soybean hypocotyls inoculated with P. sojae. A susceptible soybean cultivar (Williams) was inoculated with ~100 zoospores of each P. sojae line. Lesion lengths were measured at 36 hpi in three independent biological replicates, each of which comprised at least nine plants (**, P<0.01 compared with WT; Dunnett's test). (C) Phenotypes of lesions on etiolated soybean hypocotyls. Photographs representative of three independent experiments were taken at 36 hpi. (D/E) Callose deposition in etiolated soybean hypocotyl epidermal cells infected with P. sojae. Representative micrographs (D) of etiolated soybean hypocotyls that were inoculated with P. sojae zoospores or water (Mock) as indicated and then stained by aniline blue at 10 hpi. Bar = 50 μm. Number of callose deposits (E) per microscopic field was quantitated using ImageJ software. The data are mean ± SEM of numbers of callose deposits per microscopic field in three independent biological replicates, each of which comprised at least three soybean hypocotyls (**, P<0.01 compared with WT; Dunnett's test).