Global Analysis of the Fungal Microbiome in Cystic Fibrosis Patients Reveals Loss of Function of the Transcriptional Repressor Nrg1 as a Mechanism of Pathogen Adaptation
Fig 5
Phenazine methosulfate (PMS) or P. aeruginosa inhibits filamentation of C. albicans with standard growth morphology (Y1), but not of filamentous isolate F1.
The non-inducing condition is synthetic defined (SD) medium at 30°C and the inducing condition is SD + 5 mM N-acetylglucosamine at 37°C for 48 h. Treatments included 5 μM PMS or 100 μl of PA14 P. aeruginosa overnight culture, as indicated. Images of colonies are provided in the first and third rows, and DIC microscopy images of cells from the colony are in the second and fourth rows. Scale bar on spot image represents 2 mm, and scale bar on DIC image represents 10 μm.