Amastin Knockdown in Leishmania braziliensis Affects Parasite-Macrophage Interaction and Results in Impaired Viability of Intracellular Amastigotes
Fig 5
Subcellular localization of distinct amastins in fusion with GFP.
Promastigotes were transiently transfected with the plasmids pSP-Ama0980-GFP (A), pSP-Ama1600-GFP (B), pSP-Ama1060-GFP (C) and pSP-nGFP (D) as a control plasmid. Transfected parasites were fixed with 2% paraformaldehyde, stained with DAPI and visualized under a fluorescence microscope. Nuclear and kinetoplast DNA are shown in blue.