A Trichomonas vaginalis Rhomboid Protease and Its Substrate Modulate Parasite Attachment and Cytolysis of Host Cells
Fig 1
Subcellular localization of HA-tagged TvROMs in T. vaginalis transfectants.
Fluorescence microscopy images of indirect immunofluorescence assays (IFA) performed on T. vaginalis exogenously expressing N-terminal hemagglutinin (HA)-tagged TvROMs 1–3. (A-C) HA-TvROM1 transfectants were reacted with membrane impermeable biotin (EZ-Link-Sulfo-NHS-SS-Biotin) and IFA was then performed using formaldehyde fixation and staining with rabbit anti-HA (A-red) and a mouse anti-Biotin (B-green) antibodies. (C) Merge shows co-localization of HA-TvROM1 with the biotin-labeled T. vaginalis cell surface, scale bar = 10 μm. IFA images of HA-TvROM2 (D & E) and HA-TvROM3 (F & G) using a mouse anti-HA antibody (green) and nuclear staining using 4’-6’-diamidino-2-phenylindole (DAPI-blue). HA-TvROM2 and HA-TvROM3 show localization in a line structure adjacent to the nucleus, scale bar = 5 μm. Two juxtanuclear structures of different sizes can be observed in early and late dividing cells in HA-TvROM2 transfectants (D, see arrows). Phase images are shown on the right.