RNA-seq Brings New Insights to the Intra-Macrophage Transcriptome of Salmonella Typhimurium
Fig 2
The primary transcriptome of intra-macrophage Salmonella.
(A) Classification of 3583 Salmonella TSS during intra-macrophage proliferation (Dataset 3 in S1 Table). The TSS were categorized based on their relative-PUV, macrophage versus ESP (Materials and Methods). The red and blue circles represent the TSS that are up/down-regulated in the ∆ssrA versus InSPI2 experiment, respectively (B) The STM0854 TSS (indicated by the dotted vertical line) is a representative of a TSS highly up-regulated in macrophages. Each horizontal arrow represents the gene in scale with the whole island. Each coloured track above the island represents RNA-seq/dRNA-seq reads mapped against the genome in the corresponding conditions, visualized in the IGB browser. Each curved arrow indicates the location of a TSS; the width and height of each curved arrow is proportional to the TSS expression, based on relative PUV, macrophage versus ESP (Dataset 3 in S1 Table) (Materials and Methods).