Cryptosporidium Lactate Dehydrogenase Is Associated with the Parasitophorous Vacuole Membrane and Is a Potential Target for Developing Therapeutics
Fig 5
(A, B) Effects of pH value on the activity of CpLDH in the forward and reverse reactions. CpLDH prefers using NADH/NAD+ to convert pyruvate to lactate at conditions near neutral pH. Inset shows SDS-PAGE gel image of recombinant CpLDH (rCpLDH) used in these assays. (C—F) Activities of CpLDH on substrates, including pyruvate and lactate, and utilizing the cofactors NADH and NAD+. Insets show the Hanes-Woolf plots of the same datasets. Kinetic parameters are summarized in Table 1. S, substrate as indicated; v, velocity of the reaction. Means ± SD (n≥3) from one representative of at least three independent experiments.