LipidII: Just Another Brick in the Wall?
Fig 5
Clustering of LipidII in nonphysiological domains leads to recruitment of elongation-specific PBPs.
Under normal circumstances, LipidII (stained with fluorescent vancomycin [Van-FL], green) and RFP-PBP2A (red) co-localize at the septum (yellow in control, panel A) and the lateral wall. When LipidII is clustered into nonphysiological domains with PP-nisin, RFP-PBP2A follows LipidII in 94% of the cases when cells exhibit both LipidII and PBP2A spots (panel B, arrows, strong co-localization; arrowheads, co-localization but weak Van-FL signal). (Adapted from The localization of key Bacillus subtilis penicillin binding proteins during cell growth is determined by substrate availability, Lages MC, Beilharz K, Morales Angeles D, Veening JW, Scheffers DJ, Environmental Microbiology 15, 3272–3281 [2013], John Wiley & Sons, Inc. http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.12206/abstract.)