Comparative Life Cycle Transcriptomics Revises Leishmania mexicana Genome Annotation and Links a Chromosome Duplication with Parasitism of Vertebrates
Fig 5
Conservation of novel transcript sequences across kinetoplastid genomes.
The 936 novel L. mexicana transcripts and 7 control genes were used as queries in tblastx searches of 12 kinetoplastid genomes and the best hits were then used in a reciprocal tblastx search against the complete L. mexicana genome. The heat maps indicate the E value of the returning hits, with darker shades of blue representing lower E values. Sequences that did not return a hit are represented in red. (A) Sequences used as positive controls for conserved CDS (Gene IDs: PFR2, LmxM.16.1430; gGAPDH, LmxM.29.2980; γ-tubulin, LmxM.25.0960; SAS-6, LmxM.34.4280; RPB12, LmxM.20.0490; SmD2, LmxM.32.3190; APX, LmxM.33.0070). (B) Intergenic sequences downstream of the CDS in (A), used as negative controls. (C) Each row represents one of the 936 novel L. mexicana transcripts.