The Autophagy Receptor TAX1BP1 and the Molecular Motor Myosin VI Are Required for Clearance of Salmonella Typhimurium by Autophagy
Fig 2
TAX1BP1 localises to Salmonella that have escaped into the cytosol of infected cells.
(A) HeLa cells transfected with GFP-TAX1BP1 were infected with mCherry expressing Salmonella for 1 hour, processed for confocal immunofluorescence microscopy, and immunostained for ubiquitin. (B) HeLa cells infected for 1 hour with mCherry expressing Salmonella were immunostained for TAX1BP1 and p62 or (C) TAX1BP1 and LC3. Scale bar, 20 μm. (D) Mouse embryonic fibroblasts were infected with mCherry Salmonella for 1 hour and were immunostained for TAX1BP1 and processed for confocal microscopy. Nuclei are labelled with Hoechst (blue) (E) Still frames taken from RPE cells expressing GFP-TAX1BP1 or GFP-NDP52 infected with mCherry Salmonella imaged on a spinning disk live cell microscope. Elapsed time in min:sec is displayed.