Porphyromonas gingivalis Type IX Secretion Substrates Are Cleaved and Modified by a Sortase-Like Mechanism
Fig 9
The C-terminal peptide of P59 is modified with glycine from the growth medium.
W50WbaP was grown overnight in the (A) absence or (B) presence of 0.2 M glycine. The proteins in the CCF were subjected to SDS-PAGE separation and the band corresponding to P59 was excised and in-gel digestion was performed. Tryptic fragments were analysed by LC-MS/MS (Ion trap). Extracted ion chromatograms were plotted for m/z 729.4 (IVWSDTQWTHAN) and m/z 757.85 (IVWSDTQWTHANG). These peaks were identified as C-terminal peptide of P59 using Mascot search. * Peak corresponding to another protein.