Porphyromonas gingivalis Type IX Secretion Substrates Are Cleaved and Modified by a Sortase-Like Mechanism
Fig 7
MS analysis of intact CTD proteins shows heterogenous modification of these proteins in W50ABK*WbaP.
(A) CPG70, P59 and P27 were purified from the CF of W50ABK*WbaP and analysed by ESI-TOF for accurate molecular mass determination. Circled in blue are the expected molecular mass for each protein, except for P59. (B) W50ABK*WbaP was grown overnight in the presence of 0.2 M glycine. CPG70, P59 and P27 were purified from CF and analysed on ESI-TOF. Circled in blue are the expected molecular mass and circled in red are the expected molecular mass plus glycine (57 Da).