Inhibition of Translation Initiation by Protein 169: A Vaccinia Virus Strategy to Suppress Innate and Adaptive Immunity and Alter Virus Virulence
Fig 14
Protein 169 affects VACV virulence and immunogenicity in the intradermal model of infection.
(A) C57BL/6 mice (n = 5) were infected i.d. with 1 × 104 PFU in the ear pinna with purified viruses and the lesion sizes were monitored daily (Methods). (B) C57BL/6 mice were infected as in (A) and at the indicated times group of mice (n = 5) were killed and the viral titers in ears were determined by plaque assay. (C) C57BL/6 mice (n = 5) were infected i.d. with 169 recombinant viruses and after 28 days the mice were challenged i.n. with 5 × 106 PFU of v169 and the weight change was determined daily. Data shown are from one representative experiment (n = 2) and results are presented as the average ± SEM. Statistical analysis for all panels was performed using a two-tailed Student’s t-test, * p < 0.05.