Inhibition of Translation Initiation by Protein 169: A Vaccinia Virus Strategy to Suppress Innate and Adaptive Immunity and Alter Virus Virulence
Fig 13
vΔ169 generates better protection after challenge.
(A) BALB/c mice (n = 5) were infected i.n. with 5 × 103 PFU of the purified viruses and at day 28 the mice were challenged i.n. with 5 × 106 PFU of wild type virus and the weight change was determined daily. (B) BALB/c mice were infected as in (A) and sera were collected 28 days p.i. and assayed for neutralization of intracellular mature virus of VACV strain WR. The median value for each population is represented by a horizontal black bar. (C, D) BALB/c mice were infected as in (A) and at 28 days p.i. splenic lymphocytes were harvested and their ability to lyse uninfected YAC-1 (C) or VACV-infected P815 (D) cells was determined by chromium release assay. Data are presented as the percentage cell lysis at various effector to target (E:T) cell ratios. Data shown are from one representative experiment (n = 2) and results are expressed as the average ± SEM. Statistical analysis for all panels was performed using a two-tailed Student’s t-test, * p < 0.05.