Inhibition of Translation Initiation by Protein 169: A Vaccinia Virus Strategy to Suppress Innate and Adaptive Immunity and Alter Virus Virulence
Fig 9
Accumulated 80S monosomes do not dissociate in high salt.
(A, B) The experiment was performed as described in Fig 7 with HEK 293 Trex 169 cells induced with DOXY (1 μg/ml for 16 h). The cytoplasmic fraction was prepared in normal salt (100 mM KCl, A) or high salt (400 mM KCl, B) and analyzed on sucrose density gradients containing either 100 mM KCl (A) or 400 mM KCl (B). Data shown are from one representative experiment (n = 2). (C, D) HEK 293 Trex 169 cells were uninduced and the cytoplasmic fraction was prepared in normal salt (100 mM KCl, C) or high salt (400 mM KCl, D) and analyzed on sucrose density gradients containing either 100 mM KCl (C) or 400 mM KCl (D). Data shown are from one representative experiment (n = 2).