Inhibition of Translation Initiation by Protein 169: A Vaccinia Virus Strategy to Suppress Innate and Adaptive Immunity and Alter Virus Virulence
Fig 7
Protein 169 increases 80S monosomes and decreases polysomes.
HEK 293 Trex 169 cells were uninduced (A) or induced (B) with DOXY (1 μg/ml) for 14 h. Cytoplasmic cells lysates were prepared and resolved on 10–50% sucrose density gradients (Methods). The gradient was fractionated and fractions monitored by absorbance (A254 nm). Indicated fractions were collected and proteins were extracted, resolved by SDS-PAGE and analyzed by immunoblotting using the indicated antibodies. The positions of molecular size markers in kDa are indicated on the left and right. WCL = whole cell lysate. Data shown are from one representative experiment (n = 4).