The Hos2 Histone Deacetylase Controls Ustilago maydis Virulence through Direct Regulation of Mating-Type Genes
Fig 5
Hos2 is not downstream of the pheromone responsive Fuz7 MAPK cascade.
(A) Schematic representation of the pheromone signalling pathway via the MAP kinase cascade. The events that following pheromone-receptor recognition are numbered. (B) Induced expression of the constitutively active Fuz7 MAPK kinase allele, fuz7DD, restores conjugation tube formation in Δhos2 mutants. fuz7DD induction was performed by shifting from glucose (non-inducing) to arabinose (inducing) containing CM media. Images shown were taken 5 hours after induction. (C) Quantification of fuz7DD-induced conjugation tube formation in the indicated strains 5 hours post-induction. Mean values and SDs from three independent experiments are shown. The total number of cells counted is indicated above each column. ns denotes not statistically significant differences (t-test, p>0.05) (D-G) Expression levels of the indicated genes, relative to ppi, in FB1Pcrg1fuz7DD and the corresponding hos2 mutant in glucose and arabinose containing CM media, 5 hours post fuz7DD induction. Mean values and SDs from three independent experiments, each with three technical replicates, are shown. Values are normalised to one biological replicate of the sample with the lowest expression value, that is assigned a value of 1. One asterisk denotes p<0.05 (Duncan’s new multiple range test). ns denotes not statistically significant differences.