Intracellular Uropathogenic E. coli Exploits Host Rab35 for Iron Acquisition and Survival within Urinary Bladder Cells
Fig 6
(A) and (B) Genes involved in iron acquisition are differentially expressed by UPEC in the absence of Rab35.
si NT and si Rab35 cells were infected with UPEC at MOI 500. mRNA was isolated from either (A) cell free UPEC (represented as lab-grown UPEC) used for infecting the BEC cells or (B) UPEC isolated from infected cells at 24 h post infection and was analyzed for the expression of various genes involved in iron uptake by qRT-PCR. The values represent fold change in UPEC gene expression in (A) control (si-NT) cells relative to lab-grown UPEC or (B) Rab35-silenced cells relative to control (si-NT) cells * represents p<0.05, Values shown represent mean ± standard deviation of results of three independent experiments.