Protective mAbs and Cross-Reactive mAbs Raised by Immunization with Engineered Marburg Virus GPs
Fig 2
GP schematic and GP2-wing epitope analysis.
(A) Schematic of purified GP ectodomains used in this study. Dashed lines represent deleted regions. SS, signal sequence; MLD, mucin-like domain; IFL, internal fusion loop; TM, transmembrane domain. A red triangle indicates the furin cleavage site, numbered in red. The GP2-wing region, which is unique to MARV, is colored orange. B) MARV sequence alignment of pepscan defined epitopes for anti-GP2 wing mAbs. This region has four residues unique to strain Ravn; notably, 465E is 465K in other strains. (C) ELISA binding of GP2-wing mAbs to wild type (wt) and E465K Ravn GPΔmuc at 2 and 0.2μg/ml.