Phosphoproteomic Analysis of KSHV-Infected Cells Reveals Roles of ORF45-Activated RSK during Lytic Replication
Fig 5
The phosphorylation of several substrates with roles in translational regulation is mediated by ORF45-activated RSK.
(A) The indicated cell lines were uninduced (U) or induced (I) as described previously. Two days post-induction (dpi), cells lysates were collected and subjected to western blot analysis with the indicated antibodies. (B) Pharmacological inhibition confirms that KSHV-induced phosphorylation of eIF4B is dependent on RSK activity. iSLK.BAC16 A66F cells were uninduced (U) or induced (I) as described previously. At 46 hpi, cells were treated for 2 h with the indicated kinase inhibitor (the target of which is listed above in parentheses), then cells were harvested and lysates were analyzed by western blot with the indicated antibodies.