The Expression of Functional Vpx during Pathogenic SIVmac Infections of Rhesus Macaques Suppresses SAMHD1 in CD4+ Memory T Cells
Fig 2
The replication of Vpx defective SIV mutants is attenuated in rhesus macaque mononuclear cells.
ConA-activated rhesus PBMCs (A and B), rhesus MDM (C and D) and SupT1-R5 cells (E and F) were infected with WT and Vpx mutant SIV stocks produced in transfected 293T cells. The indicated cell cultures were infected with equivalent amounts of virus inocula, based on particle-associated 32P -RT activity (approximately 5 × 106 cpm) and progeny virion production was monitored by measuring the RT activity released into the culture medium. The results in panels A and B, are shown as mean +/—s.e.m (n = 4). The parametric unpaired t test was performed using PRISM software. The significant p values (* p<0.05, ** p<0.01) for WT versus the X-del and X-Q76A Vpx mutants refer to the single time points at peak virus production. A representative result from at least two experiments is shown in panels C and D.