Characterization of Arabidopsis Transcriptional Responses to Different Aphid Species Reveals Genes that Contribute to Host Susceptibility and Non-host Resistance
Fig 7
ROS levels during host and non-host interactions with aphids.
Leaves exposed to different aphid species were incubated with the dye DCFH-DA (dichlorodihydro-fluorescein diacetate) to compare reactive oxygen species (ROS) levels during host and non-host interactions. Images were taken 3, 6, 12 and 24 hours after aphid exposure using a laser confocal microscope and processed in ImageJ to generate graph bars representing relative fluorescence to the control treatment (no aphids). Graph indicates fluorescence ratios of leaf samples exposed to aphids compared to a no-aphid control (C). Average relative ratios are based on 5 different leaf samples per treatment. Three independent replicated experiments, with 5 leaf samples per treatment per replicate, were performed with similar results. Graph shows results of one of the two replicates. The additional replicate is shown in S9 Fig.