Varicella Viruses Inhibit Interferon-Stimulated JAK-STAT Signaling through Multiple Mechanisms
Fig 2
IFN-induced nuclear translocation of STAT is blocked in SVV-infected cells.
(A) TRFs were infected with SVV.eGFP (ratio 10:1) and at 48 hours p.i., the cells were stimulated with 5000 U/ml uIFN for 40 minutes. Cells were fixed with 4% paraformaldehyde, permeablized and stained for STAT2 using a specific antibody. SVV infection (green) and STAT2 localization (red) were visualized by immunofluorescence microscopy. Insert shows enlargement of the outlined area. (B) Cytoplasmic and nuclear fractions were isolated from mock- and SVV.eGFP (ratio 5:1)-infected Vero cells that were stimulated with 5000 U/ml uIFN for 40 minutes at 48 hours p.i.. The fractions were analyzed for STAT2 expression by SDS-PAGE and western blotting. An antibody directed against ORF62 confirmed productive SVV-infection. Fraction purity was confirmed using GAPDH (cytosolic) and p84 (nuclear). Results from one of three independent experiments is shown.