Recovery of Recombinant Crimean Congo Hemorrhagic Fever Virus Reveals a Function for Non-structural Glycoproteins Cleavage by Furin
Fig 5
CCHFV M segment polyprotein processing.
(A) Signal peptide (SP) and predicted transmembrane domains are indicated in yellow. Arrows indicate locations of cleavage motifs recognized by mammalian convertases. The RSKR motif (boxed) was mutated to ASKA to block processing. (B) Signal peptidase (SPase) cleavages result in production of Gn and Gc precursors (PreGn and PreGc). Binding regions of the anti-glycoprotein antibodies used in this study (7F5, Gn tail, 11E7, and 6C11) are also represented. (C) Mature glycoprotein products (GP160/85, GP38, Gn, and Gc) resulting from mammalian convertase cleavage. (D) S1P cleavage is required for the production of GP160/85 and Gn, while GP38 requires cleavage by both S1P and furin-like PCs. An unidentified mammalian convertase is required for PreGc maturation to Gc.