Manipulation of the Xanthophyll Cycle Increases Plant Susceptibility to Sclerotinia sclerotiorum
Fig 5
Impact of Sclerotinia invasion on ABA biosynthesis.
(A) Effect of Sclerotinia invasion on the expression of ABA biosynthesis genes. The expression of target genes was determined by qPCR. (B) Immuno-cytochemical localization of ABA in longitudinal sections of Arabidopsis leaves. Bars = 0.2 mm. (C) Measurement of ABA levels in Sclerotinia-inoculated leaf regions with an ELISA Kit. (D) A2 mutant invasion caused leaf yellowing surrounding the necrotic lesions. Images were captured at 24 h after inoculation with A2 mutant. (E) Quantitative analysis of the content of ABA in the yellowing regions. (F) Mutation of VDE alleviated the decrease in ABA levels in Sclerotinia-infected npq1-2 leaves. WT, wild-type Sclerotinia; A2, oxalate-deficient A2 mutant. (**), Student’s t-test significant at P< 0.01; (*), P< 0.05. Different letters indicate statistically significant differences (Duncan’s multiple range tests; P< 0.05). Data are average of three replicates± SE.