Discovery of a Small Non-AUG-Initiated ORF in Poleroviruses and Luteoviruses That Is Required for Long-Distance Movement
Fig 5
Infection of A. thaliana infiltrated leaves.
A. Northern blot hybridization of RNA extracted from A. thaliana leaves agroinfiltrated with the wild-type TuYV (WT) or the mutants TuYV-3aAUG (AUG), TuYV-3aAGC (AGC), or TuYV-3aFLAG (FLAG). Mock, mock-inoculated control; gRNA, genomic RNA; sgRNA1, subgenomic RNA1, LC, loading control of ethidium bromide stained ribosomal RNA. Each sample corresponds to a mixture of leaves from 3 individual plants. B. and C. Western blot analysis of proteins extracted from the same infected leaf samples. Proteins were blotted on the same membrane and incubated with antibody (@) specific to P3a (B), CP, P4 and RTD protein (C). The blots were then cut in two for the figure. Two different exposures (short and long) are shown for P3a detection. LC, loading control of proteins stained on the membranes by Ponceau red.