Establishment of HSV1 Latency in Immunodeficient Mice Facilitates Efficient In Vivo Reactivation
Fig 5
Acute and latent gene expression in HD infected B6-Rag trigeminal ganglia.
qRT-PCR analysis of RNA from Tg collected from acute (day 5) PBS (blue bars) or IVIG treated (red) and latent (day 60) IVIG treated (black) HD B6-Rag mice are shown as fold-change relative to LAT expression (A-D) for Immediate Early (A), Early (B), Early/Late (C) and Late (D) genes, and delta-Ct (dCt) values normalized to GAPDH expression for Early, Early/Late and Late genes (E, F, and G, respectively). Tg sections obtained from latently infected LD, HD, and a HD Rag mouse showing signs of HSE were processed for RNA-FISH using a LAT RNA probe (H). The blue signal stains for LAT, the green signal comes from neuronal cytoplasmic lipofuscin (aggregates) autofluorescence. The dotted lines outline the nuclei. Wide-field imaging. Scale bar = 10 μm. (I) GAPDH normalized dCt values for LAT expression in HD Rag mice determined using RT-PCR (I). Statistical analysis is described in methods.