Compartmentalized Replication of R5 T Cell-Tropic HIV-1 in the Central Nervous System Early in the Course of Infection
Fig 5
All viruses required high levels of CD4 for efficient entry, indicative of primarily being selected for replication in CD4+ T cells.
Single-cycle infection of HIV-1 Env-pseudotyped reporter viruses on CD4lowCCR5high 293-Affinofile cells [28]. Receptor expression was measured as follows: CD4low = 972 receptors/cell, CD4high = 72,041 receptors/cell, CCR5high = 15,636 receptors/cell. The data are averaged from triplicate wells for each of 2 to 3 env clones that were generated per indicated amplicon. Amplicons were selected for cloning to represent different portions of the phylogenetic tree. Subjects with no evidence of CNS compartmentalization (i.e. equilibrated) are shown in panel (A); and subjects with CNS compartmentalization at one or more time points are in panel (B). Longitudinal time points are indicated (T1, T2, T3, etc.) and samples with marked pleocytosis are noted (*).