Characterization of Metabolically Quiescent Leishmania Parasites in Murine Lesions Using Heavy Water Labeling
Fig 2
Rates of RNA turnover in cultured and intracellular Leishmania stages.
Kinetics of 2H-labeling of RNA ribose in (A) cultured parasite stages (Prolog, Prostat, Amaaxenic) (B) amastigotes isolated from infected J774 macrophages (AmaMø) and (C) amastigotes isolated from BALB/c lesions (Amalesion). The fraction of new molecules (Y-axis) was calculated from the level of 2H-enrichment in Rib relative to maximum labeling observed in each parasite stage after long term labeling. Inset boxes shows estimated RNA turnover (t1/2 in days) in each stage. D. Comparative rates of RNA turnover in different Leishmania developmental stages.