Super-Resolution Imaging of ESCRT-Proteins at HIV-1 Assembly Sites
Fig 5
Super-resolution imaging of CHMP2A at HIV-1 assembly sites.
HeLa cells were transfected with HIV:HIVmCherry and endogenous CHMP2A was detected by immunostaining 14–15 h post transfection. HeLa cells were imaged using TIRFM. (A) Left panel. A merged time-projected TIRF image of HIVmCherry (magenta) and CHMP2A (green) prior to STORM analysis. Middle panel. A zoomed-in image of the selected CHMP2A cluster highlighted in grey in the left panel colocalizing with an HIV assembly site. Right panel. The corresponding drift-corrected STORM image of the CHMP2A cluster. Scale bars: 500 nm. (B) The size distribution of all CHMP2A structures colocalizing with HIVmCherry with an average cluster size (FWHM) of 56 ± 12 nm. (C) Size distribution of all non-colocalizing CHMP2A clusters in cells co-expressing HIVmCherry with an average cluster size (FWHM) of 59 ± 20 nm.