Lymph-Node Resident CD8α+ Dendritic Cells Capture Antigens from Migratory Malaria Sporozoites and Induce CD8+ T Cell Responses
Figure 7
Summarizes Figs. 1–6 as a model.
Sporozoite-mediated delivery of malaria antigens to the DLNs is required for CD8+ T cell priming. Anopheline mosquitoes inject sporozoites into the skin of their mammalian host, a tissue populated by epidermal Langerhans cells, langerin+ dermal DCs (CD207+), and langerin- dermal DCs (CD207-). Following sporozoite inoculation into the dermis, a proportion of sporozoites enter the DLNs via the afferent lymphatic vessel and are found in close association with CD169+ macrophages lining the subcapsular and medullary sinuses. LN-resident DCs directly sample and phagocytose sporozoites and sporozoite-derived CS particles within the LN parenchyma. Sporozoite-specific CD8+ T cells are primed by LN-resident CD8α+ DCs residing along the cortical ridge and the T cell zone of the DLN as early as 8 hours after immunization. Printed with permission from Heidi Sinsel, 2014.