Lymph-Node Resident CD8α+ Dendritic Cells Capture Antigens from Migratory Malaria Sporozoites and Induce CD8+ T Cell Responses
Figure 6
CD8α+ DCs are necessary for the presentation of sporozoite antigens to CD8+ T cells.
A and B. WT and Batf3−/− mice received 1×106 CFSE-labeled OT-1 cells 1 day prior to ID injection of 2×104 irradiated P. berghei CS5M sporozoites. DLNs were collected 3 days post-inoculation. A. Representative CFSE profiles of OT-1 cells from 1 of 3 similar experiments. B. Expansion of OT-1 cells in the DLN; mean ± SEM, n = 7/group. Data are representative of 3 similar experiments. C. 0.5–1×104 naive OT-1 cells were transferred to WT and Batf3−/− mice 1 day before immunization via 20 irradiated P. berghei CS5M-infected mosquito bites. Responses were examined 8, 14, and 17 days post-inoculation. Proportion of CD8+ T cells of OT-1 origin after P. berghei CS5M-infected mosquito bites. Data are pooled from 2 similar experiments mean ± SEM; n = 9/group (day 8) and n = 6/group (day 14 and 17).